Journal: bioRxiv
Article Title: Adult Hox gene expression promotes periosteal stem cell maintenance and mediates reprogramming in a regionally restricted manner
doi: 10.1101/2022.05.15.492027
Figure Lengend Snippet: ( A ) Representative FACS plots of the PSPC lineage output 7 days after transduction of PP1 cells with LV-GFP or LV-Hoxa10/GFP . Infected (GFP + ) and uninfected (GFP − ) cells were evaluated separately. ( B ) Flow cytometric analysis on a CellTrace TM positive control (dye administration 30 minutes before analysis) and on a CellTrace TM negative control (no dye administration) was used to categorize cells as low- or high-cycling using the indicated gating strategy. A sample of LV-Hoxa10/GFP -infected PP1 cells after six days of in vitro administration of CellTrace TM was used as a representative sample. ( C ) The number of cells seven days after transduction of PP1 cells with LV-GFP or LV-Hoxa10/GFP with or without 10 μg/μl Mitomycin C treatment2. n = 4. **** p < 0.0001. Two tailed Student’s t-test. Error bars are SEM.
Article Snippet: CellTrace TM Far Red Cell Proliferation Kit (ThermoFisher Scientific: C34564) was used per manufacturer’s instructions.
Techniques: Transduction, Infection, Positive Control, Negative Control, In Vitro, Two Tailed Test